![]() ![]() The results of this study demonstrate that CGB scaffolds are biocompatible with hUC-MSCs and that granular CGB scaffolds covered with hUC-MSCs are expected to generate new advances for future treatment of traumatic brain injury.Ĭrown Copyright © 2012. BDNF released from CGB scaffold promoted neuronal differentiation of NSCs and led to significant differences in differentiation rate and average neuron perimeter compared with the control group. In this review, we provide a comprehensive overview of CS structure and its physicochemical and biological properties, which have been employed as printing materials to fabricate 3D scaffolds. Chitosan is especially attractive as a bone scaffold material because it supports the attachment and proliferation of osteoblast cells as well as formation of. Chitosan (CS) has been used extensively as a biopolymer for biomaterials used in bone tissue renewal 39, 40. The CGB scaffolds released BDNF in a uniform profile, whereas the CB scaffolds only released BDNF during the first 3 days. Chitosan, a biodegradable, naturally occurring polymer, has drawn considerable attention in recent years as scaffolding material in tissue engineering and regenerative medicine. There were on average more cells on the CGB scaffold on the first day than on any other day sampled (P < 0.05). There were no significant differences in diameter and porosity of individual CGB scaffolds (P > 0.05). Finally, we investigated whether the released BDNF can induce NSC to differentiate into neurons. The amount of BDNF released from CGB over a 30 day period was determined by ELISA. Prior to this, the diameter and porosity of CGB scaffolds were measured. The viability of hUC-MSCs adhered to the CGB scaffold was determined by digesting with 0.25% trypsin and evaluating with the cell counting kit-8 (CCK-8). Fluorescent nuclear staining (Hoechst 33342) was employed to determine the attachment of the hUC-MSCs to CGB scaffolds on the 1st, 3rd, 7th and 10th day of co-culture. hUC-MSCs were co-cultured with the CGB scaffold. The collagen/chitosan scaffold was more effective to promote and accelerate L929 cell proliferation, particularly for scaffolds containing 30 of chitosan. The chitosan scaffold based on immobilization of BDNF by genipin (GP) as a crosslinking agent referred to hereafter as a CGB scaffold was prepared by freezing-drying technique. This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF blended chitosan scaffolds on neural stem cell differentiation with the aim of contributing alternative methods in tissue engineering for the treatment of traumatic brain injury (TBI).
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